Detection of Colistin Resistance in Gram Negative Pathogens: A One Year Cross-sectional Study in a Tertiary Care Centre in Northeast India

International Journal of Medicine and Public Health,2022,12,4,175-179.DOI:10.5530/ijmedph.2022.4.32Published:January 2023Type:Original ArticleDetection Colistin Resistance in Gram Negative Pathogens: A One Year Cross-sectional Study a Tertiary Care Centre Northeast IndiaDeepayan Biswas, Wihiwot Valarie Lyngdoh, Sheryl Lanong, Clarissa Jane Prithwis Bhattacharyya, Nari Mary Lyngdoh Deepayan Biswas1, Lyngdoh2, Lanong2, Bhattacharya3, Lyngdoh3 1Department Microbiology, NEIGRIHMS, Mawdiangdiang, Shillong, Meghalaya, INDIA 2Department INDIA. 3Department Anesthesiology Critical Care, Abstract:Background: Indiscriminate use colistin for gram-negative infections has led to increase incidence resistance. The problem nosocomial especially caused by multi-drug-resistant bacteria (MDR-GNB), particularly Acinetobacter baumannii, Pseudomonas aeruginosa Klebsiella pneumoniae is matter great concern. This study was conducted find out the prevalence resistant isolates from patients attending outdoor patient department (OPD), those admitted wards Intensive care unit centre North-East India. Materials Methods: Clinical samples obtained were processed using standard microbiological methods. showing resistance Kirby- Bauer’s disc diffusion method included further subjected MIC (minimum inhibitory concentration) testing VITEK-2 system followed confirmation Broth microdilution method. Results: observed 26 1040 values varied 8 ≥ 32 μg/ml. Majority them belong species highly ß-lactams, aminoglycosides, fluoroquinolones. Conclusion: highlights an increasing trend amongst multidrug (MDR) warranting routine screening guide appropriate therapy future use. Keywords:Broth microdilution, Colistin, Disc diffusion, Multidrug organisms, VITEK-2View:PDF (346.91 KB)